Full, or so, Curriculum Vitae

Anatoly V. Chernyshev, PhD (chemistry)

 

Education
1994–1999: PhD (Physical Chemistry), Saratov State University, Russia; Institute of Physiology and Biochemistry of Plants and Microorganisms, Russian Academy of Sciences, Russia. Dissertation: “Physical chemistry of impact of metal ions on the metabolism of bacterium Azospirillum brasilense
1989–1994: MSc (Organic Chemistry), Saratov State University, Russia

Technical skills
Spectroscopy (UV-Vis, IR, NMR, fluorescent).
Mass spectrometry (conventional and isotope-ratio MS).
Chromatography (GC, LC, HPLC, TLC).
Vacuum systems & gaseous substances; Schlenk lines.
Single channel electrophysiology.
Protein purification, enzyme assays.

Employment

February 2015 — Present
Scientist, Analytica Laboratories Ltd. (New Zealand). Stable isotope analysis (IRMS, CRDS) in food and environmental science. Kinetic modeling of manuka honey chemistry.

August 2012 — June 2014
Senior Consultant in Research & Development for Science, Innovation Dept., the Research Council of the Sultanate of Oman.

  • Development of strategy and mechanisms of scientific innovation in Oman.
  • Reviewing of science intensive innovation proposals.
  • Drafting of national programs on: complex processing of palms (date and coconut), pharmaceutical development of Boswellia sacra resin (frankincense), industrial cultivation of guar beans, pottery manufacturing, cold weapon (khanjar) metallurgy.
  • Drafting of Oman research program in the Antarctic.

September 2008 — July 2012
Assistant Professor of Chemistry at Dept. of Biological Sci. & Chemistry, University of Nizwa, Sultanate of Oman. Lecture and laboratory courses: Fundamentals of Chemistry, Organic Chemistry, Petroleum Chemistry, Biophysical Chemistry, Environmental Chemistry, Food Chemistry.

September 2003 — August 2008
Research associate at Chemistry Dept., University of Iowa, Iowa City, USA.
Study of heavy kinetic isotope effects in nitrogenase enzyme reactions; study of flavin-dependent thymidylate synthase.

January 2007 — May 2007
Visiting Assistant Professor at Chemistry Dept., University of Iowa, Iowa City, USA. Lecture course on physical chemistry.

March 2001 — August 2003
Research associate at Physiology Dept., Loyola University, Chicago, USA.
Experimental study of peptide proton channels (gramicidin A); molecular mechanic modeling of proton current.

August 94 — March 2001
Research associate, Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences, Saratov, Russia. Study of glutamine synthetase enzyme; study of biogeochemical formation of mineral struvite. Postgraduate MSc thesis supervisor (chemistry).

June 2000 — March 2001
Senior Chemical Ecology Engineer at the Scientific Center "EMOS", Saratov, Russia.
Ecological expeditions and field studies; environmental impact assessment of operations in gas and oil deposit territories in West Siberia.

September 1995 — December 2000
Lecturer, Department of Chemistry, Saratov State University, Russia. Lecture course “Structure and functions of biomacromolecules”.

Grant and contract support

Title                                         The Recovery of Silver From Veladero Ore Using
a Microwave  Process
Source                                     Barrick Gold Inc.
Funding period                       April 2009 – July 2010

 

Title                                         ALS Biomarker Based on Isotope Distributions
Source                                     Private foundation (Prize4Life.org)
Funding period                       February 2008 – January 2009

 

Intellectual property development and awards

IdeaConnection awards (2015): Measuring Chemical Concentration by LC-MS Without Characterized Reference Standards; Controlling Vertebrate Pests in New Zealand.

InnoCentive Challenge awards (2005-2013): calculation of birefringence in liquid crystals; development of the biomarker for amyotrophic lateral sclerosis; development of chemical mechanisms of cellulose pyrolysis; identification of new promising protein targets for drugs; development of a process of improving UV resistance of coatings containing metal ions; development of a technology preventing a preservative degradation (http://www.innocentive.com).
1997, 1998          The Soros graduate student (cumulative award for the postgraduate studies http://www.soros.org).

Service
Faculty service
Member of the following committees at Dept. of Biological Sciences and Chemistry, University of Nizwa: Laboratories and library committee; Students training and field trips committee; Strategic planning and quality assurance committee.

Membership in Professional Organizations

2015–present       New Zealand Institute of Chemistry

2003–present       The American Chemical Society
2003–present       The International Union of Pure and Applied Chemistry (IUPAC;          Affiliate member)

Public Service
2008–2014       Grant Committee Member (Culture, Basic and Social Sciences sector), Oman Research Council (www.trc.gov.om)


Publications


Refereed journal papers

  1. Chernyshev A. О ядовитых медах и их пользе. Химия и жизнь (Of Poisonous Honeys and their Use. Chemistry and Life, in Russian)- 2017.- N. 8.- P. 28-32
  2. Chernyshev A. The C4 Sugar Test: AOAC vs. Screening Method. The New Zealand Beekeeper - 2017.- V. 25.- N. 7.- P. 11-13
  3. Chernyshev A. Of Tutu and Bugs. Chemistry in New Zealand - 2017.- V. 81.- N. 1.- P. 19-23
  4. Howse S., Chernyshev A. Forecasting the change in manuka honey is now a reality. The New Zealand Beekeeper - 2015.- V. 23.- N. 9.- P. 47-48
  5. Chernyshev A., Melghit K. Characterization of a mineral coating of Dyerophytum indicum plant//Z. Naturforsch. C - 2015.- V. 70(3-4).- P. 59-64
  6. Wang Z., Chernyshev A., Koehn E. M., Manuel T. D., Lesley S. A., Kohen A. Oxidase activity of a flavin-dependent thymidylate synthase //FEBS J.- 2009.- V. 276.- P. 2801-2810
  7. Chernyshev A.V., Fleischmann T., Koehn E., Lesley S.A., Kohen A. The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS)// Chem. Comm.- 2007.- P. 2861-2863 (DOI 10.1039/b700977a; also featured in Chemical Biology. 2007.- N 8).
  8. Chernyshev A.V., Fleischmann T., Kohen A. Thymidyl Biosynthesis Enzymes as Antibiotic Targets (Mini-review)// Applied Microbiology and Biotechnology.- 2007.- V. 74.- P. 282-289 (DOI 10.1007/s00253-006-0763-1).
  9. Chernyshev A. V., Cukierman S. Proton Transfer in Gramicidin Water Wires in Phospholipid Bilayers. Attenuation by Phosphoethanolamine.// Biophys. J.- 2006.- V. 91 - P. 580-587.
  10. Chernyshev A. V., Armstrong K. M., Cukierman S. Proton Transfer in Gramicidin Channels is Modulated by the Thickness of Monoglyceride Bilayers.// Biophys.  J. - 2003- V. 84 - P. 238 -250.
  11. Chernyshev A. V., Pomes R., Cukierman S. Kinetic isotope effects of proton transfer in aqueous and methanol containing solutions, and in gramicidin A channels.//Biophys. Chem.- 2003. – V. 103. – P. 179–190.
  12. Chernyshev A. V., Cukierman S. A thermodynamic view of activation energies of proton transfer in various gramicidin A channels.// Biophys. J.- 2002.- V. 82.- P. 182-192.
  13. Kamnev A.A., Antonyuk L.P., Colina M., Chernyshev A.V., Ignatov V.V. Physicochemical investigation of a microbially produced modification of magnesium-ammonium orthophosphate//Monatsh. Chem. (Chemical Monthly).- 1999 - V. 130 -  P. 1431-1442.
  14. Kamnev A.A., Ristic M., Colina M., Antonyuk L.P., Chernyshev A.V., Ignatov V.V. Comparative spectroscopic study of intact cells and cell membranes of the bacterium Azospirillum brasilense //In: Spectroscopy of Biological Molecules: Modern Trends (P. Carmona, R. Navarro, A. Hernanz, eds.).-Dordrecht: Kluwer Acad. Publ.- 1997.- P. 313-314.
  15. Kamnev A.A., Renou-Gonnord M.-F., Antonyuk L.P., Colina M., Chernyshev A.V., Frolov I., Ignatov V.V. Spectroscopic characterization of the uptake of essential and xenobiotic metal cations in cells of the soil bacterium Azospirillum brasilense //Biochem. Mol. Biol. Int.- 1997.- V. 41.- N 1.- P. 123-130.
  16. Kamnev A.A., Ristic M., Antonyuk L.P., Chernyshev A.V., Ignatov V.V. Fourier transform infrared spectroscopic study of intact cells of the nitrogen-fixing bacterium Azospirillum brasilense //J. Mol. Struct.- 1997 - V. 408/409 - P. 201-205.
  17. Kamnev A.A., Antonyuk L.P., Chernyshev A.V., Ignatov V.V., Colina de Vargas M. Spectroscopic characterization of mineral crystals produced by the bacterium Azospirillum brasilense//Fresenius J. Anal. Chem.- 1996 - V. 355 - P. 739-741.
  18. Antonyuk L. P., Kamnev A. A., Chernyshev A. V., Ignatov V. V. The formation of struvite crystals during cultivation of the soill bacterium Azospirillum brasilense//Doklady Akademii Nauk (Proceedings of the Russian Academy of Sciences).-1996.- V. 350.-N3.- P. 421-423.

Book sections:

  • Chernyshev A.V. “Structure and functions of biomacromolecules”. In:  Lecture courses and practices in bioorganic chemistry (Schegolev S. Yu., Astakhova L.N. Eds.) 2-nd ed., Saratov State University Press, 2000 (In Russian).

Conference presentations:

  1. Chernyshev A. C4 Sugar Testing in Honey. Detecting Sugar Adulteration by Isotope Analysis. Apiculture New Zealand, Rotorua, New Zealand, July 9–11, 2017.
  2. Chernyshev A., Grainger M., Braggins T. Predicting the Composition of Manuka Honey. Apiculture New Zealand, Rotorua, New Zealand, June 19–21, 2016.
  3. Chernyshev A. Validation of Laser Cavity Ringdown Spectrometry (CRDS) for 13C Isotopic Analysis in Honey. Stable Isotope Network for New Zealand Workshop, Dunedin, New Zealand, August 27–28, 2015.
  4. Chernyshev A., Chernysheva O. Application of Kinetic Isotope Effects (KIE) for Development  of a Biomarker for Amyotrophic Lateral Sclerosis (ALS). Biomarkers for Brain Disorders: Challenges and Opportunities. Wellcome Trust Scientific Conferences, Hinxton, Cambridge, UK, February 27 – March 2, 2011
  5. Wang Z., Chernyshev A.V., Fleischmann T., Lesley S. A., Kohen A. The Competition between O2 and 5,10-Methylene-5,6,7,8-Tetrahydrofolate (CH2THF) in the Oxidative Half Reaction of Flavin-Dependent Thymidylate Synthase (FDTS). XXVII Midwest Enzyme Chemistry Conference, Chicago, USA, September 29, 2007.
  6. Chernyshev A.V., Koehn E., Fleischmann T., Wang Z., Lesley S.A., Kohen A. Thermotoga Flavin Dependent Thymidylate Synthase (FDTS): Implications for Oxygen Metabolism. Thermotoga 2007 Workshop, La Jolla, USA, July 9-10, 2007. http://www.calit2.net/newsroom/article.php?id=1132
  7. Fleischmann T., Chernyshev A.V., Kohen A. Mechanistic aspects of flavin-dependent thymidylate synthetase. XXVI Midwest Enzyme Chemistry Conference, Chicago, USA, September 30, 2006.
  8. Sra A., Chernyshev A., Kohen A. 15N Kinetic Isotope Effects in Nitrogenase Reaction. Gordon Research Conference “Nitrogen Fixation”, New London, NH, USA, June 20-25, 2004.
  9. Chernyshev A., Armstrong K., Cukierman S. Bilayer Thickness Affects H+ Current through the Native and Covalently Linked Gramicidin A Channels. 47th Biophysical Society Annual Meeting, San Antonio, USA, March 1-5, 2003 // Biophys. J. - 2003 - V. 84 - No 2 - P. 94a.
  10. Chernyshev A., Cukierman S. Temperature dependence of H+, D+ and K+ current through the native and covalently linked gramicidin A channels. 46th Biophysical Society Annual Meeting, San Francisco, USA, February 23-27, 2002.
  11. Chernyshev A. V., Fomina O. R., Kutuzova E. V., Moksin S. A., Konoplyantseva N. A. Role of the ions of f-elements in biological systems. Electrochemical and colorimetric study on interaction of a bacterial glutamine synthetase and bovine serum albumine with lanthanides and uranyl (VI) ions//Abstr. Eur. Conf. «Bacterial-Metal/Radionuclide Interaction: Basic Research and Bioremediation».- 2-4 Dec. 1998 - Rossendorf, Germany.- P. 67-68.
  12. Chernyshev A. V., Fomina O. R., Kutuzova E. V., Moksin S. A. Catalysis performed by a bacterial glutamine synthetase in the presence of ions of s-, p- and d-elements//Ibid.- P. 60-62.
  13. Chernyshev A. V. A Matter Study at the Microscopic Level Using Combinatorics// Abstr. 27th Int. School of Crystallography `Implications of Molecular and Materials Structure for New Technologies', Erice, Italy, 28 May-7 June 1998, P10.
  14. Chernyshev A. V., Kutuzova E. V., Kutuzov S. V., Fomina O. R.,Konoplyantseva N. A. An electrochemical investigation of glutamine synthetase reactions//Abstr. 7th Eur. Conf. on Electroanalysis.- 24-28 May 1998 - Coimbra, Portugal.- P. 43.
  15. Chernyshev A. V. A Combinatorial Approach for Investigation of Assembled Systems//Abstr. 2nd Ann. European Conf. on Combinatorial Technologies, 4-6 Feb. 1998, Montreux, Switzerland, P. 5.
  16. Chernyshev A. V. A theoretical study on the long-range protein-protein and cell-cell interaction.//Mol. Biol. Cell -  1996 - V. 7 - P. 1648.
  17. Kutuzova E. V., Fomina O. R., Konoplyantseva N. A., Chernyshev A. V. An electrochemical investigation of the kinetics of glutamine synthetase purified from Azospirillium brasilense.// Mol. Biol. Cell - 1997 - V. 8 - P 1648.
  18. Fomina O. R., Chernyshev A. V., Kutuzova E. V., Konoplyantseva N. A. Electrochemical investigation of the kinetics of a bacterial glutamine synthetase//The FASEB J.- 1997- V. 11.- N 9.- P. A1140.
  19. Chernyshev A. V. Combinatorial Analysis of Complex Systems//The FASEB J. 1997. V. 11. N9. P. A838.
  20. Moksin S. A., Chernyshev A. V. Interrelationships between Primary and Quaternary Structures of Heteropolymers Originated from Different Alphabets//The FASEB J. 1997. V. 11. N9. P. A838.
  21. Kamnev A.A., Antonyuk L.P., Colina M., Chernyshev A.V., Ignatov V.V. Assimilation of essential and xenobiotic metal cations by the associative nitrogen-fixing bacterium Azospirillum brasilense//Abstr. 8th Int. Congr. of Bacteriology and Applied Microbiology Division, IUMS.- 18-23 Aug. 1996.- Jerusalem, Israel.- P. 104.
  22. Kamnev A.A., Ristic M., Antonyuk L.P., Chernyshev A.V., Ignatov V.V. Fourier transform infrared spectroscopic studies of intact cells of the nitrogen-fixing bacterium Azospirillum brasilense and some model compounds related to its metabolism//Abstr. XXIII Eur. Congr. on Molecular Spectroscopy.- 25-30 Aug. 1996.- Balatonfüred, Hungary.- P. 122.
  23. Kamnev A.A., Chernyshev A.V., Antonyuk L.P., Colina M., Ignatov V.V. Involvement of metal cations by the soil bacterium Azospirillum brasilense in the processes of its metabolism //Abstr. 26th Int. Symp. on Environmental Analytical Chemistry.- 9-12 Apr. 1996 - Vienna, Austria.- P. 35.
  24. Chernyshev A. V., Kutuzova E. V., Moksin S. A., Antonyuk L. P., Ignatov V. V. The behaviour of glutamine synthetase from the soil bacterium Azospirillum brasilense with ions of lanthanides //Abstr. 7th Int. Symp. «Nitrogen Fixation with Non-Legumes».- 16-21 Oct. 1996 - Faisalabad, Pakistan.- P. 16.
  25. Chernyshev A. V., Moksin S. A., Kutuzova E. V., Antonyuk L. P., Ignatov V. V. Interaction of glutamine synthetase from the soil bacterium Azospirillum brasilense with ions of lanthanides and uranyl (VI) ion// Abstr. Int. Symp. on Subsurface Microbiology.- 15-21 Sept. 1996 - Davos, Switzerland.- P. 80.
  26. Chernyshev A. V., Kosterina N. V., Antonyuk L. P., Ignatov V. V. Influence of triphenylsubstituted pirilium, thiopirilium and selenopirilium salts on the catalytic activityof a bacterial glutamine synthetase//Abstr. 17th Int. Conf. on the Organic Chemistry of Sulphur.- 7-12 July, 1996.- Tsukuba, Japan.- P. 29.
  27. Chernyshev A. V. Thermodynamical Attractors of Live Systems as a Possible Key to Elucidation of New Drugs//Abstr. 24th Int. School of Crystallography `Experimental and Computational Approaches to Structure Based Drug Design', Erice, Italy, 9-19 May 1996, P10.
  28. Bespalova L. A., Chernyshev A.V., Antonyuk L.P., Ignatov V.V. Glutamine synthetase of Azospirillum brasilense: influence of the activating metal on the enzyme properties //Abstr. 10th Int. Congr. on Nitrogen Fixation.- 28 May-3 June, 1995.- St. Petersburg, Russia - P. 113.
  29. Kamnev A.A., Antonyuk L.P., Chernyshev A.V., Ignatov V.V., Colina de Vargas M. Spectroscopic characterization of mineral crystals produced by the bacterium Azospirillum brasilense //Abstr. XXIX Colloquium Spectroscopicum Internationale.- 27 Aug.- 1 Sept. 1995.- Leipzig, Germany.- P. 492.
  30. Chernyshev A. V. Alternating Structures in Biology//Abstr. 3rd Int. Symp. on Bioorganic Chemistry, Dagomys, Russia, 17-23 Sept. 1995, P. 29.
  31. Chernyshev A. V. Effects of levelling of Fermi energy in multimeric protein associations //Abstr. 3rd IUBMB Conf. «Molecular recognition».- 23-27 Apr. 1995.- Singapore.-P. 115.
  32. Chernyshev A. V., Antonyuk L. P., Bespalova L. A., Ignatov V. V. Metal specificity of Azospirillum brasilense glutamine synthetase//Ibid.-P. 156.

Publications about my research

  1. Chernyshev A. V. Chemical Reaction. Notes on 2011 Declared Year of Chemistry by IUPAC, H! Times of Oman, January 7, 2011, P. 7
  2. Shatalova N. Trials for good. Russian scientist is in the InnoCentive winners list, SEARCH (POISK), Weekly newspaper of Russian Academy of Sciences//N12 (878) March 24, 2006 (in Russian; http://www.achernyshev.com/poisk.html)
  3. Rodrigo I.M. Se buscan investigadores, Tiempo de Hoy, April 20, 2007 (in Spanish; http://www.tiempodehoy.com/default.asp?idpublicacio_PK=50&idioma=CAS&idnoticia_PK=41558&idseccion_PK=764)
  4. Prize4Life ALS Biomarker Track 1 Award Summaries, Prize4Life Public Release (http://www.prize4life.org/uploaded_files/biomarker_challenge_track_1_summaries_86cLkC.pdf)
  5. UI chemists' DNA biosynthesis discovery could lead to better antibiotics, EurekAlert, Public Release: April 16, 2009 (http://www.eurekalert.org/pub_releases/2009-04/uoi-ucd041609.php)

 

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